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  • Title: Spin label studies of the essential sulfhydryl group environment in chicken liver fructose-1,6-bisphosphatase.
    Author: Zeidan H, Han P, Johnson J.
    Journal: FEBS Lett; 1985 Nov 18; 192(2):294-8. PubMed ID: 2998872.
    Abstract:
    The local environment of the essential sulfhydryl groups in chicken liver fructose-1,6-bisphosphatase has been investigated by ESR techniques using a series of iodoacetamide spin labels, varying in chain length between the iodoacetate and nitroxide free radical group. The ESR spectrum of spin-labeled chicken liver fructose-1,6-bisphosphatase showed that the sites of labeling were highly immunobilized when the enzyme was chemically modified by spin label iodoacetate, suggesting that the sulfhydryl groups of the protein are in a small, confined environment. From the change in the ESR spectra of these nitroxides as a function of chain length, we conclude that the sulfhydryl group is located in a cleft approx. 10.5A in depth.
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