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  • Title: Metabolomic Approach for the Authentication of Berry Fruit Juice by Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry Coupled to Chemometrics.
    Author: Zhang J, Yu Q, Cheng H, Ge Y, Liu H, Ye X, Chen Y.
    Journal: J Agric Food Chem; 2018 Aug 01; 66(30):8199-8208. PubMed ID: 29989408.
    Abstract:
    Berry fruit juice, which is represented by blueberry and cranberry juice, has become increasingly popular due to its reported nutritional and health benefits. However, in markets, adulteration of berry fruit juice with cheaper substitutes is frequent. In the present study, a metabolomic approach for authentication of berry fruit juices by liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) was established. The global characterization of the berry fruit metabolome by information dependent acquisition directed LC-MS/MS coupled to a peak mining workflow by isotope pattern matching was reported. Targeted metabolomics analysis of known juice biomarkers, such as flavonoids, anthocyanins, etc. exhibited a good separation of berry fruit juices from adulterant juices. Moreover, untargeted metabolomics analysis was carried out and subjected to chemometrics analysis. Discrimination of blueberry juice, cranberry juice, and its adulterant apple juice and grape juice was obtained by principal component analysis-discriminant analysis. Eighteen characteristic markers discriminating berry fruit juice and its adulterants were selected by comparison of marker abundances in different juice samples. Identification of characteristic markers was accomplished by elemental formula prediction and online database searches based on accurate MS information. These results suggested that the combination of untargeted and targeted metabolomics approach has great potential for authentication of berry fruit juice.
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