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Title: [Analysis of the characteristics of lymphocyte subsets in peripheral blood among coal worker's pneumoconiosis patients].
Author: Lu J, Li J, Han QY, Lu XX, Hu Y, Chen HC, Hu J.
Journal: Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi; 2018 May 20; 36(5):353-356. PubMed ID: 29996380.
Abstract:
Objective: To preliminary analysis of the characteristics of lymphocyte subsets in peripheral blood among 135 cases of coal worker's pneumoconiosis patients in Huainan mining area. Methods: The peripheral bloods of 135 cases of coal worker's pneumoconiosis patients and 112 cases of health examiners were collected. Flow cytometry was used to detect peripheral blood lymphocytes, T cell subsets and CD4(+)CD25(+) regulatory T cells. Results: Compared with the control group, CD64 index of granulocytes and lymphocytes was slightly higher. The total T cells (CD3(+)) increased in peripheral blood, CD4(+) expression was reduced and CD8(+) expression was increased in infection group, CD4(+)/CD8(+) ratio was inverted, the differences between the infection group and the control group were statistically significant (P<0.05) . There were significantly fewer NK (nature killer) and B cells, significantly more double negative T cells (DNT, CD3(+)CD4(-)CD8(-)) than the control group (P<0.05) . There was no statistically significant difference of CD4(+)CD25(+)CD127(+low)、CD4(+)CD25(+)CD127(+hi) and the ration of Treg/CD4(+)CD25(+)CD127(+hi) protein expression in peripheral blood in two groups (P>0.05) . Conclusion: The analysis of peripheral blood lymphocyte and subgroup is an ideal index to monitor the immune status of coal worker's pneumoconiosis patients. It has theoretical significance for studying the immune mechanism of pneumoconiosis and guiding clinical treatment. 目的： 分析煤工尘肺病患者外周血中淋巴细胞及其亚群的特点。 方法： 采集135例煤工尘肺病患者（煤工尘肺组）及112例正常体检者（对照组）的外周血，用流式细胞仪检测外周血中淋巴细胞、T细胞亚群以及CD4(+)CD25(+)调节性T细胞等。 结果： 煤工尘肺组外周血中的粒细胞和淋巴细胞CD64指数高于正常对照，总T细胞（CD3(+）)升高，CD4(+)T细胞减低，CD8(+)T细胞升高，CD4(+)/CD8(+)比例倒置，与正常对照组比较，差异有统计学意义（P<0.05）；煤工尘肺组NK、B细胞减低，双阴性T细胞（double negative T cells，DNT）（CD3(+)CD4(-)CD8(-）)明显升高，差异有统计学意义（P<0.05）；煤工尘肺组外周血CD4(+)CD25(+)调节性T细胞（CD4(+)CD25(+)regulatory T cells，CD4(+)CD25(+)Treg）中CD4(+)CD25(+)+CD127(+low)和CD4(+)CD25(+)CD127(+hi)百分比降低，与正常对照组的差异无统计学意义（P>0.05）。 结论： 外周血中淋巴细胞及亚群分析可用于监测煤工尘肺病患者免疫功能。.

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