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  • Title: The expression of cytokines IFN-γ, IL-4, IL-17A, and TGF-β1 in peripheral blood and follicular fluid of patients testing positive for anti-thyroid autoantibodies and its influence on in vitro fertilization and embryo transfer pregnancy outcomes.
    Author: Lu H, Huang Y, Xin H, Hao C, Cui Y.
    Journal: Gynecol Endocrinol; 2018 Nov; 34(11):933-939. PubMed ID: 29996685.
    Abstract:
    The aim of this work was to study the expression of the cytokines IFN-γ, IL-4, IL-17 A, and TGF-β1 in peripheral blood and follicular fluid (FF) of patients positive for antithyroid autoantibodies (ATA+) with normal thyroid gland function and the influence of these autoantibodies on in vitro fertilization and embryo transfer (IVF-ET) pregnancy outcomes. Nineteen patients were in the ATA+ group, and 27 patients tested negative for anti-thyroid autoantibody (ATA-). Blood samples were drawn from the two groups of patients on the oocyte retrieval day and the 5th and 14th days of transplantation; in addition, FF was extracted on the oocyte retrieval day from both groups of patients and tested through enzyme-linked immunosorbent assay (ELISA) for IFN-γ, IL-4, IL-17 A, and TGF-β1. For the ATA+ group, the concentration of IFN-γ increased whereas the concentration of TGF-β1 decreased in peripheral blood on the oocyte retrieval day (p < .05); the concentration of IL-4 decreased in peripheral blood on the 5th and 14th days of transplantation for the ATA+ group (p < .05); further, the concentration of IL-17 A increased whereas that of TGF-β1 decreased in FF (p < .05). The ratio of IL-17 A/TGF-β1 in the ATA+ group significantly increased in FF and peripheral blood on the oocyte retrieval day and the 14th day of transplantation (p < .05). The ratio of IL-17 A/TGF-β1 in FF of the pregnant patients was significantly lower than in the non-pregnant patients (p < .05). The findings suggested that the ratio between pro-inflammatory and anti-inflammatory cytokines was adversely affected; therefore, adverse pregnancy outcomes of patients with ATA+ undergoing IVF-ET treatment may be attributed to immunological mechanisms. 摘要 这项工作的目的是研究甲状腺功能正常的抗甲状腺自身抗体阳性（ATA +）患者细胞因子IFN-γ, IL-4, IL-17A和TGF-b1在外周血和卵泡液（FF）中的表达, 以及探究这些自身抗体对体外受精和胚胎移植（IVF-ET）妊娠结局的影响。ATA +组中共计19名患者, 对照组为检测为抗甲状腺自身抗体（ATA-）阴性的27位患者。在取卵日, 移植后的第5天和第14天从两组患者中抽取血样, 另外, 在两组患者的取卵日提取卵泡液, 并通过酶联免疫吸附测定（ELISA）测量IFN-γ, IL-4, IL-17A和TGF-β1的表达水平。ATA +组在取卵日IFN-γ浓度增加, 外周血中TGF-β1浓度降低（p <.05）；ATA +组移植后第5天和第14天外周血中IL-4浓度降低（p <.05）; 在卵泡液中, IL-17A浓度增加而TGF-β1浓度降低（p <.05）。 在取卵日和移植后第14天, ATA +组卵泡液和外周血中IL-17A / TGF-β1的比例显著增加（p <.05）。 妊娠患者卵泡液IL-17 A / TGF-β1的比例显著低于非妊娠患者（p <.05）。 研究结果表明, ATA +患者促炎细胞因子和抗炎细胞因子比例受到了不利影响; 接受IVF-ET治疗的ATA +患者的不良妊娠结局可归因于免疫机制。.
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