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Title: [Icariin alleviates lipid peroxidation after spinal cord injury in rats].
Author: Ren XS, Ding W, Yang XY.
Journal: Nan Fang Yi Ke Da Xue Xue Bao; 2018 Jun 20; 38(6):711-715. PubMed ID: 29997094.
Abstract:
OBJECTIVE: To assess the effects of intragastric administration of icariin on lipid peroxidation after spinal cord injury in rats. METHODS: Seventy-two healthy adult male SD rats were randomized equally into icariin group, control group and sham-operated group. In the control and icariin groups, spinal cord injury was induced using modified Allen's method, and the rats in the sham-operated group underwent laminotomy without damaging the spinal cord. Immediately after the surgery, the rats in icariin group were subjected to intragastric administration of icariin (100 mg/kg), and those in the control and sham-operated groups received an equal volume of saline in the same manner once a day. At 24 h after the operation, malondialdehyde (MDA) content was detected using thiobarbituric acid method, superoxide dismutase (SOD) activity was measured with xanthine oxidase method, and the water content in the spinal cord was measured using dry-wet weight method. At 48 h after the operation, the ultrastructure of the spinal cord was observed with transmission electron microscopy and scored using Kaptanoglu scoring method. The motor function of the rats was assessed using BBB scoring at 7, 14, 21 and 28 days after the operation. RESULTS: At 24 h after the operation, MDA content was significantly higher in the control group and icariin group than in the sham-operated group, and was significantly lower in icariin group than in the control group (P<0.05); SOD activity was significantly higher in icariin group than in the control group, and was both significantly lower than that in the sham-operated group (P<0.05). At 48 h after operation, the water content and ultrastructure score of the spinal cord were the highest in sham-operated group (P<0.05), and were significantly lower in icariin group than in the control group (P<0.05). At all the time points of measurement, the BBB scores were significantly lower in the control and icariin groups than in the sham-operated group (P<0.05), and were significantly higher in icariin group than in the control group (P<0.05). CONCLUSION: Icariin can significantly reduce MDA content, increase SOD activity, and ameliorate lipid peroxidation, spinal cord edema, and histopathological damage of the spinal cord to improve motor function of rats with spinal cord injury. 目的: 研究淫羊藿苷对大鼠脊髓损伤后脂质过氧化的影响。方法: 72只健康成年清洁级雄性SD大鼠按随机数字表法分为淫羊藿苷组、对照组及假手术组3组，每组24只。对照组和淫羊藿苷组采用改良Allen法制作脊髓损伤模型，假手术组仅切开椎板不损伤脊髓。术后即刻淫羊藿苷组给予淫羊藿苷（100 mg/kg）灌胃，对照组和假手术组给予等量生理盐水灌胃，1次/d。术后24 h采用硫代巴比妥酸法检测丙二醛（MDA）含量，黄嘌呤氧化酶法检测超氧化物歧化酶（SOD）活性；采用干湿重法检测脊髓组织的含水量；术后48 h采用透射电镜观察脊髓组织超微结构，并采用Kaptanoglu评分法进行超微结构评分；术后7、14、21、28 d采用BBB评分法评定大鼠运动功能。结果: 术后24 h对照组和淫羊藿苷组MDA含量显著高于假手术组，淫羊藿苷组低于对照组，差异有统计学意义（P < 0.05）；对照组和淫羊藿苷组SOD活性显著低于假手术组，淫羊藿苷组高于对照组，差异有统计学意义（P < 0.05）。术后48 h，对照组和淫羊藿苷组脊髓组织含水量、超微结构评分均显著高于假手术组，淫羊藿苷组均显著低于对照组（P < 0.05）。术后各时间点对照组和淫羊藿苷组大鼠BBB评分均低于假手术组，差异有统计学意义（P < 0.05）；淫羊藿苷组大鼠BBB评分高于对照组，差异有统计学意义（P < 0.05）。结论: 淫羊藿苷能够明显降低脊髓损伤后的MDA含量，升高SOD的活性，减轻脂质过氧化、脊髓水肿和脊髓的组织病理学损伤，改善脊髓损伤大鼠的运动功能，有效地保护脊髓组织和神经作用。

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