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  • Title: [Deletion of late genes of the prophage lambda].
    Author: Matvienko NI, Troianovskaia IN, Zheleznaia LA, Iarchuk OB.
    Journal: Mol Biol (Mosk); 1985; 19(6):1457-65. PubMed ID: 3001508.
    Abstract:
    The deletions in tandem prophage lambda appear with high frequency (to 10%) in rec A- strain of Escherichia coli. The deletions were shown by marker rescue and hybridization of fragments of DNA on nitrocellulose filters with nick-translated phage lambda DNA localized only in prophage area. Right and left att sites are not involved. The majority of defective lysogens had all regulatory regions and deletions of late structural genes. These strains may be used for construction of the host-vector systems with the strongest promoter p'R of phage lambda.
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