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  • Title: Upregulation of circular RNA hsa_circ_0007534 predicts unfavorable prognosis for NSCLC and exerts oncogenic properties in vitro and in vivo.
    Author: Qi Y, Zhang B, Wang J, Yao M.
    Journal: Gene; 2018 Nov 15; 676():79-85. PubMed ID: 30017736.
    Abstract:
    Emerging evidence documented the key functions of circular RNAs (circRNAs) in various malignancies. Nevertheless, the research relevant to the clinical value and functions of circRNAs in non-small cell lung cancer (NSCLC) is still very limited. In this study, we performed qRT-PCR to assess the levels of hsa_circ_0007534 in NSCLC tissues and cell lines and evaluated its clinical significance using Fisher's exact test, Kaplan-Meier analysis and Cox regression analysis. Additionally, loss-of-function and gain-of-function experiments were performed to detect whether hsa_circ_0007534 could affect cell proliferation, apoptosis, metastatic properties and epithelial-mesenchymal transition (EMT) in A549 and H1299 cells. Further xenograft study was carried out to validate the in vitro data. The results indicated that hsa_circ_0007534 was up-regulated in both NSCLC tissue samples and cell lines and this up-regulation is linked to lymph node invasion and advanced TNM stage. Hsa_circ_0007534 could also function as an unfavorable prognostic indicator for the patients with NSCLC. For the part of functional assays, down-regulation of hsa_circ_0007534 suppressed cell growth, migratory and invasive capacities; facilitating cell apoptosis in A549 and H1299 cells. Conversely, up-regulation of hsa_circ_0007534 caused the opposite biological behaviors. What's more, animal experiments validated the oncogenic role of hsa_circ_0007534. Ultimately, the present study indicates that hsa_circ_0007534 might be a potential NSCLC-associated prognostic/therapeutic target.
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