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  • Title: Selective and sensitive fluorescent and colorimetric chemosensor for detection of CO32- anions in aqueous solution and living cells.
    Author: Karuk Elmas ŞN, Ozen F, Koran K, Gorgulu AO, Sadi G, Yilmaz I, Erdemir S.
    Journal: Talanta; 2018 Oct 01; 188():614-622. PubMed ID: 30029421.
    Abstract:
    A new colorimetric and fluorescent chemosensor for visual determination of carbonate ions was developed by the microwave assisted solvent free synthesis of 7,8-dihydroxy-3-(4-methylphenyl) coumarin (DHMC). The structural characterization of DHMC was confirmed by microanalysis and spectroscopy methods (MALDI-TOF, FT-IR, 1H NMR, 13C NMR, and 2D HETCOR). The binding behaviors of DHMC were investigated towards various anions by UV-vis and fluorescence spectroscopy. DHMC showed a selective and sensitive fluorometric and colorimetric responses towards carbonate ion over other anions. The detection limit of CO32- was found to be 1.03 µM. Moreover, the fluorescence imaging in living cells suggests that DHMC has a great potential in the biological imaging application. It has been demonstrated that DHMC can be used as a rapid and reliable sensor for the determination of carbonate anion in a variety of practical applications.
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