These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [New method for the synthesis and cloning of cDNA].
    Author: Borovkov AIu.
    Journal: Genetika; 1986 Jan; 22(1):172-4. PubMed ID: 3005107.
    Abstract:
    A simple method for cloning cDNA has been suggested. The plasmid pUC18 was digested with Pst1. A plasmid primer for cDNA synthesis was prepared by dT tailing with terminal transferase. After synthesis of cDNA, dG tails were added and then 3' ends blocked with rG. The plasmid was digested with Kpn1 and dC tails were added, after which annealing took place and RNA:DNA hybrids were used for Escherichia coli transformation. The efficiency of approx. 10(4) transformants per microgram of starting mRNA has been obtained.
    [Abstract] [Full Text] [Related] [New Search]