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  • Title: Separation of nuclear cAMP independent protein kinases NI and NII from their chromosomal protein substrates and enzyme inhibitors by the use of a casein-phosvitin-Sepharose column.
    Author: Delpech M, Levy-Favatier F, Kruh J.
    Journal: Anal Biochem; 1986 Jan; 152(1):100-6. PubMed ID: 3006541.
    Abstract:
    A casein-phosvitin-Sepharose chromatography column allows separation of nuclear protein kinases from their chromosomal phosphoprotein substrates and from at least some protein kinase inhibitors in a single step. The additional step of passing the eluted material through a partially hydrolyzed, dephosphorylated casein-Sepharose column separates the two protein kinases, NI and NII, from each other.
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