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  • Title: The development of nanobiocatalysis via the immobilization of cellulase on composite magnetic nanomaterial for enhanced loading capacity and catalytic activity.
    Author: Han J, Luo P, Wang Y, Wang L, Li C, Zhang W, Dong J, Ni L.
    Journal: Int J Biol Macromol; 2018 Nov; 119():692-700. PubMed ID: 30071227.
    Abstract:
    In this study, graphene oxide (GO) decorated with 4arm‑PEG‑NH2 (molecular weight (MW) 5 K or 10 K) was constructed on magnetic Fe3O4, denoted as GO@Fe3O4@4arm‑PEG‑NH2. The morphology, structure and magnetic property of GO@Fe3O4@4arm‑PEG‑NH2 were characterized by Fourier transform infrared (FTIR), vibrating-sample magnetometer (VSM), thermogravimetric analysis (TGA), transmission electron microscopy (TEM), X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS) in details. The saturation loading capacity of GO@Fe3O4@4arm‑PEG‑NH2 (MW 5 K and 1 K) carriers toward cellulase was 429 and 575 mg/g, respectively. Additionally, the immobilized cellulase had exhibited enhanced thermostability, storability and reusability than free enzyme. The two kinds of immobilized cellulose (MW 5 K and 10 K) retained 57% and 60% of its initial activity after 3 h at 70 °C, and retained 47% and 50% of its initial activity after 30 days' storage at room temperature. After eight times reuse, immobilized cellulose (MW 5 K and 10 K) retained 40% and 45% of its initial activity, respectively. In practical application, glucose generated by the saccharification with the immobilized cellulase was much higher than free enzyme (immobilized enzyme is kept at 2.04-2.83 times of the free enzyme), when the loading amount of enzyme was 2-8 mg, indicating the potential of the prepared biocatalyst.
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