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  • Title: Quantification of active ingredients in Potentilla tormentilla by Raman and infrared spectroscopy.
    Author: Mazurek S, Fecka I, Węglińska M, Szostak R.
    Journal: Talanta; 2018 Nov 01; 189():308-314. PubMed ID: 30086923.
    Abstract:
    The most important active compounds present in Potentilla tormentilla rhizomes were quantitatively determined on the basis of Raman, attenuated total reflection (ATR) and diffuse reflectance mid- and near- infrared spectra (DRIFTS) collected for the untreated plant material. Partial least-squares (PLS) models were constructed utilizing vibrational spectra and the results of reference high-performance liquid chromatography analyses. Applying Raman spectroscopy, total polyphenols, tannins, ellagitannins, procyanidins, agrimoniin, 3-O-galloylquininc acid and catechin were simultaneously quantified in tormentil rhizomes, with the relative standard errors of prediction in the 2.0-4.9% range for both calibration and validation sets. These error ranges were found to be slightly higher for infrared techniques and amounted to 2.7-6.5%.
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