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  • Title: Translocation of cAMP-dependent protein kinase to the nucleus during development of Dictyostelium discoideum.
    Author: Woffendin C, Chambers TC, Schaller KL, Leichtling BH, Rickenberg HV.
    Journal: Dev Biol; 1986 May; 115(1):1-8. PubMed ID: 3009250.
    Abstract:
    The distribution of the catalytic and regulatory subunits of the cAMP-dependent protein kinase between cytoplasm and nucleus was determined during the development of Dictyostelium discoideum. In vegetative amoebae approximately 2% of the subunits were in the nucleus. During development there was an approximately 5-fold increase in total soluble cAMP-dependent protein kinase and a 15- to 30-fold increase of enzyme in the nuclear fraction. There was a reverse translocation from nucleus to cytoplasm, when Tipped Aggregates were disrupted and the resultant amoebae incubated in single-cell suspension. The addition of cAMP to these single-cell suspensions brought about the reentry of the subunits into the nucleus. The findings are discussed in relation to the potential role of the cAMP-dependent protein kinase in the regulation of mRNA and protein synthesis.
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