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  • Title: [Endonucleolysis of chromatin in thymocytes of irradiated and non-irradiated rats].
    Author: Ivannik BP, Sin'kova RV, Konov AV, Riabchenko NI.
    Journal: Radiobiologiia; 1986; 26(2):162-6. PubMed ID: 3010369.
    Abstract:
    It was shown that in conditions optimal for Ca/Mg endonuclease, chromatin endonucleolysis in the nuclei and thymocytes occurs due to internucleosome fragmentation of DNA. Irradiation activates chromatin degradation in thymocytes washed by a buffer containing 0.25 M sucrose, 10 mM tris-HCl, pH 7.2, 3 mM MgCl2, and does not influence this process in thymocytes washed by 10 mM tris-HCl, pH 7.2, 3 mM MgCl2.
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