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  • Title: The assay of ATP-sulfurylase.
    Author: Hommes FA, Moss L.
    Journal: Anal Biochem; 1986 Apr; 154(1):100-3. PubMed ID: 3010765.
    Abstract:
    An assay method for ATP-sulfurylase is described, based on the incorporation of 35SO4(2-) into adenosine-5'-phosphosulfate (and 3-phosphoadenosine-5'-phosphosulfate) and the separation of these compounds by HPLC. Since the enzyme is easily inactivated at temperatures above 20 degrees C during the assay, the reaction time should not exceed 10 min.
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