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  • Title: Highly Sensitive and Selective Colorimetric Detection of Methylmercury Based on DNA Functionalized Gold Nanoparticles.
    Author: Xie ZJ, Bao XY, Peng CF.
    Journal: Sensors (Basel); 2018 Aug 15; 18(8):. PubMed ID: 30111699.
    Abstract:
    A new colorimetric detection of methylmercury (CH₃Hg⁺) was developed, which was based on the surface deposition of Hg enhancing the catalytic activity of gold nanoparticles (AuNPs). The AuNPs were functionalized with a specific DNA strand (HT7) recognizing CH₃Hg⁺, which was used to capture and separate CH₃Hg⁺ by centrifugation. It was found that the CH₃Hg⁺ reduction resulted in the deposition of Hg onto the surface of AuNPs. As a result, the catalytic activity of the AuNPs toward the chromogenic reaction of 3,3,5,5-tetramethylbenzidine (TMB)-H₂O₂ was remarkably enhanced. Under optimal conditions, a limit of detection of 5.0 nM was obtained for CH₃Hg⁺ with a linear range of 10⁻200 nM. We demonstrated that the colorimetric method was fairly simple with a low cost and can be conveniently applied to CH₃Hg⁺ detection in environmental samples.
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