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  • Title: Purification of Clathrin-Coated Vesicles from Adult Rat Brain.
    Author: Giovedì S.
    Journal: Methods Mol Biol; 2018; 1847():1-11. PubMed ID: 30129005.
    Abstract:
    Here, we describe a purification protocol for isolating clathrin-coated vesicles (CCVs) from adult rat brain by using differential centrifugation coupled with Ficoll-sucrose and D2O-sucrose density gradient centrifugation and an additional linear sucrose step gradient at the end to separate CCVs from contaminating membranes present in the crude microsomal fraction.
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