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  • Title: An alteration in tubulin expression detected in SV40 transformed 3T3 cells.
    Author: Raxworthy MJ, Shaw JR, Marriott MS.
    Journal: Cell Biol Int Rep; 1986 Jul; 10(7):555-63. PubMed ID: 3015424.
    Abstract:
    Tubulin expression was analysed in normal and simian virus-40 (SV40) transformed 3T3 cells by two-dimensional polyacrylamide gel electrophoresis and immunoblotting studies using monoclonal antibodies raised to alpha- and beta-tubulin subunits. The ratio of alpha- to beta-tubulin recognised was calculated for both cell lines and found to shift from 2.50 in normal cells to 0.52 in virally transformed cells. beta-Tubulin was thereby shown to be the predominant subunit in SV40-transformed 3T3 cells in contrast to normal 3T3 cells.
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