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  • Title: Identification and characterization of recD, a gene affecting plasmid maintenance and recombination in Escherichia coli.
    Author: Biek DP, Cohen SN.
    Journal: J Bacteriol; 1986 Aug; 167(2):594-603. PubMed ID: 3015881.
    Abstract:
    We isolated mutations that reduce plasmid stability in dividing cell populations and mapped these mutations to a previously undescribed gene, recD, that affects recombination frequency and consequently the formation of plasmid concatemers. Insertions of the transposable element Tn10 into recD resulted in increased concatemerization and loss of pSC101 and ColE1-like replicons during nonselective growth. Both concatemer formation and plasmid instability in recD mutants require a functional recA gene. Mutations in recD are recessive to recD+ and map to a small region of the Escherichia coli chromosome located between recB and argA. Although the recD locus is distinct from loci encoding the two previously identified subunits of the RecBC enzyme, mutations in recD appear to affect the exonuclease activity of this enzyme.
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