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Title: [Intervention effect of Modified Dahuang Zhechong Granule on epididymal morphological changes in experimental varicocele rats].
Author: Wang QS, Jiang Y, Chen HT, Wang YL, Zhou B, Dai B, Yang DF, Qin YY, Li F, Hu EY.
Journal: Zhonghua Nan Ke Xue; 2018 Mar; 24(3):241-246. PubMed ID: 30161311.
Abstract:
OBJECTIVE: To explore the effect of Modified Dahuang Zhechong Granule (MDZG) on the development and maturation of epididymal sperm in experimental varicocele (VC) rats. METHODS: Sixty SD male rats were randomly divided into six groups of equal number, sham operation, VC model, Aescuven forte, and low-, medium- and high-dose MDZG. The model of left VC was made by the Turner method in all the rats except those of the sham operation group, followed by treatment with 0.9% normal saline for the animals in the sham operation and VC model groups, Aescuven forte tablets at 54 mg per kg of the body weight for those in the Aescuven forte group, and MDZG at 0.6, 1.2 and 2.4 g/ml for those in the low-, medium- and high-dose MDZG groups, all administered intragastrically qd for 8 successive weeks. Then, all the rats were sacrificed and their left epididymides harvested for examination of the quality of the epididymal sperm and the local microscopic and ultrastructural changes of the epididymal tissue. RESULTS: The VC model rats showed significant apoptosis of the epididymal sperm cells, interstitial edema, microvascular dilatation, degeneration and degeneration of the epithelial cells, degeneration of some principal cells and basal cell vacuoles, and immature spermatids in the lumen. Sperm motility was significantly increased in the Aescuven forte and low-, medium- and high-dose MDZG groups as compared with the VC models (P <0.01). Both sperm concentration and motility were markedly higher in the high-dose MDZG than in the Aescuven forte group (P <0.05). Remarkable apoptosis of epididymal sperm cells was observed in the microenvironment of sperm development in the VC models, which exhibited no statistically significant difference from that in the rats of the medium- and high-dose MDZG groups. CONCLUSIONS: Experimental varicocele induced local apoptosis of epididymal sperm cells, interstitial edema and microvascular dilatation in the rat epididymis, while Modified Dahuang Zhechong Granule could improve the stability of epididymal sperm maturation and contribute to their development. 目的：探讨实验性精索静脉曲张大鼠附睾管精子发育成熟的改变及加味大黄蟅虫颗粒对附睾精子成熟的影响。方法： 60只SD雄性大鼠随机分为模型组、假手术组、迈之灵组及加味大黄蟅虫颗粒低、中、高剂量组，每组10只。除假手术组外，各组按Turner方法制作左侧精索静脉曲张大鼠模型。造模完成后，假手术组及模型组大鼠均给予生理盐水；迈之灵组给予迈之灵片54 mg/kg体重药量溶于生理盐水中；加味大黄蟅虫颗粒低、中、高剂量组分别予以含生药0.6、1.2、2.4 g/ml加味大黄蟅虫颗粒水冲剂。各组皆按1 ml/100 g体重灌胃，每日1次，连续8周。给药8周后全部处死各组实验大鼠, 取出左侧附睾，检测附睾精子质量及局部显微超微结构的改变，并行组间对比。结果：模型组大鼠附睾管局部上皮细胞退化变性，部分主细胞及基细胞空泡变性，管腔内见各级未成熟精子细胞。与模型组［(12.77±6.25)%］比较，加味大黄蟅虫颗粒中［(32.23±6.55)%］、高［(39.39±8.53)%］剂量组和迈之灵组［(31.10±7.77)%］大鼠附睾精子活率升高（P<0.01）。与迈之灵组比较，加味大黄蟅虫颗粒高剂量组附睾精子活率［(31.10±7.77)% vs (39.39±8.53)%］及浓度［(10.69±4.92)×10⁶/ml vs (36.37±7.09)×10⁶/ml］明显增高（P<0.05）。结论：实验性精索静脉曲张大鼠附睾管局部细胞凋亡明显、间质水肿、微血管扩张，加味大黄蟅虫颗粒能提高附睾培育精子成熟的稳定性，为精子的发生发育创造有利的条件。.

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