These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: 125I-FK 33-824: a selective probe for radioautographic labeling of mu opioid receptors in the brain.
    Author: Moyse E, Pasquini F, Quirion R, Beaudet A.
    Journal: Peptides; 1986; 7(2):351-5. PubMed ID: 3016685.
    Abstract:
    The selectivity of the Met-enkephalin analog FK 33-824 (FK) for mu opioid receptors has been, over the years, a matter of controversy. We report here pharmacological and radioautographic data demonstrating that at nanomolar concentrations. 125I-FK interacts exclusively with mu sites. (1) Specific binding of 125I-FK to rat striatal membranes is totally inhibited by mu- and/or delta-preferring ligands according to monovalent, Michaelian kinetics, with a potency proportional to the affinity of competing drugs for mu receptors. (2) Unlabeled FK competes only at high concentration with the delta-selective ligand 3H-DPLPE and according to the same kinetics as the mu-selective agonist DAGO. (3) 125I-FK generates the same regional radioautographic labeling pattern as 3H-DAGO. We conclude that when used at nanomolar concentrations 125I-FK constitutes a selective probe for the radioautographic detection of mu opioid receptors at both light and electron microscopic levels.
    [Abstract] [Full Text] [Related] [New Search]