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  • Title: Detect molecular spectral features of newly developed Vicia faba varieties and protein metabolic characteristics in ruminant system using advanced synchrotron radiation based infrared microspectroscopy: A preliminary study.
    Author: Rahman MM, Refat B, Zhang H, Zhang W, Yu P.
    Journal: Spectrochim Acta A Mol Biomol Spectrosc; 2019 Jan 05; 206():413-420. PubMed ID: 30170176.
    Abstract:
    Recently, advanced synchrotron radiation-based Fourier transform infrared microspectroscopy (SR-IMS) has been developed as a rapid, direct, non-destructive and bioanalytical technique. To date, there has been very little application of this technique to study the molecular structure make-up in pulse seeds. Thus, the objectives of this study were to detect the interactive association between protein molecular structure and nutrient availability of newly developed Vicia faba varieties. Two different varieties of faba beans (CDC Snowdrop = low-tannin variety; vs. FB9-4 = high-tannin variety) were selected for this study. The molecular spectra data were collected by using SR-IMS. The ratio of both amide I to II area and height were higher (P < 0.01), while the ratio of α-helix to β-sheet was lower (P < 0.05) in CDC Snowdrop compared to FB9-4. The crude protein (CP) content and the predicted truly digestible nutrients as well as the bioenergy values did not vary between two varieties. The CDC Snowdrop had exhibited a higher (P < 0.01) rapidly degradable CP fraction (75.99 vs. 71.45% on CP) and a lower (P < 0.01) moderately degradable CP fraction (19.43 vs. 22.85% on CP), resulting in a higher (P < 0.01) rumen degradable protein and a lower (P < 0.01) rumen undegradable protein content than that of FB9-4 variety. However, the total supply of digestible rumen undegraded feed protein was higher (P < 0.05) in FB9-4 than CDC Snowdrop. Strong positive correlations were found between the ratio of α-helix to β-sheet and CP contents (R = 0.86, P < 0.01) as well as the truly digestible CP contents (R = 0.83, P < 0.01); respectively. In conclusion, the results of this study reveal that the protein are metabolized differently between different type of faba bean varieties and the advanced SR-IMS molecular spectroscopy can be used to rapidly delineate protein molecular structure motifs along with their nutritive value in ruminant livestock system.
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