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  • Title: Identification of a rat liver cDNA and mRNA coding for the 28 kDa gap junction protein.
    Author: Heynkes R, Kozjek G, Traub O, Willecke K.
    Journal: FEBS Lett; 1986 Sep 01; 205(1):56-60. PubMed ID: 3017758.
    Abstract:
    By screening of a rat liver cDNA library with complex and deoxyinosine containing oligonucleotide probes a cDNA clone was isolated and shown by sequencing to code for the amino-terminal half of the rat liver 28 kDa gap junction protein. The insert hybridized to a 1.9 kb species from rat and mouse liver poly(A)+ RNA in Northern blot analysis. In embryonic mouse hepatocytes the amount of the 1.9 kb mRNA increased 3-fold between 24 and 96 h in culture. This correlates with the previously described increase of the 28 kDa gap junction protein under these conditions.
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