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  • Title: Requirement of the Escherichia coli dnaA gene product for plasmid F maintenance.
    Author: Kline BC, Kogoma T, Tam JE, Shields MS.
    Journal: J Bacteriol; 1986 Oct; 168(1):440-3. PubMed ID: 3020005.
    Abstract:
    There are DnaA protein-binding sites in at least one F origin of replication, and only potentially leaky dnaA(Ts) mutations had ever been used in previous studies indicating that F replication was independent of the dnaA gene product. Here we show that an Escherichia coli dnaA::Tn10 host which does not make a dnaA gene product cannot sustain autonomous or integrated F plasmid maintenance.
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