MEDLINE/PubMed Journal Browser Search

Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

Title: MicroRNA Dysregulation and Steroid Hormone Receptor Expression in Uterine Tissues of Rats with Endometriosis during the Implantation Window.
Author: Cai H, Zhu XX, Li ZF, Zhu YP, Lang JH.
Journal: Chin Med J (Engl); 2018 Sep 20; 131(18):2193-2204. PubMed ID: 30203794.
Abstract:
BACKGROUND: Estrogen receptor (ER) and progesterone receptor (PR) are involved in endometriosis, but the involvement of microRNAs (miRNAs) is unknown. The aim of the study was to explore the correlation between miRNA and ER/PR in uterine tissues of rats with endometriosis during the implantation window. METHODS: Twenty female Sprague-Dawley rats were randomized in three groups: endometriosis (n = 7), fat tissue control (n = 6), and normal (n = 7) groups. The female rats were mated and sacrificed on day 5 (implantation). Uterine tissues were obtained for hematoxylin-eosin staining, immunohistochemistry, and miRNA expression. Reverse transcription polymerase chain reaction (RT-PCR) was used to validate the expression of rno-miR-29c-3p, rno-miR-34c-5p, rno-miR-141-5p, rno-miR-24-1-5p, and rno-miR-490-5p. RESULTS: The 475 miRNAs were found to differentially express between the endometriosis and normal control groups, with 127 being upregulated and 348 being downregulated. Expression of five miRNAs (rno-miR-29c-3p, rno-miR-34c-5p, rno-miR-141-5p, rno-miR-24-1-5p, and rno-miR-490-5p) were validated by RT-PCR and found to be differentially expressed among the three groups. Expression of ER and PR proteins (immunohistochemistry) in the glandular epithelium and endometrial stroma was significantly different among the three groups (all P < 0.05). Five miRNAs were involved in pathways probably taking part in implantation and fertility. CONCLUSIONS: The results suggested that miRNAs, ER, and PR could play important roles in the embryo implantation period of rats with endometriosis. These miRNAs might play a role in endometrial receptivity in endometriosis. MicroRNA表达谱与甾体激素受体在内异症大鼠种植窗期的表达研究摘要背景：研究发现雌激素受体 (ER)和孕激素受体(PR)表达于子宫内膜异位症，但关于microRNAs (miRNAs)在内异症当中的作用至今未知。本研究的目的是探索miRNA和ER/PR之间的相互调控联系在内异症大鼠种植窗期子宫当中的作用。 方法：20只雌性SD大鼠随机分成三组：内异症组(n=7)、脂肪组织对照组(n=6)和空白对照组(n=7)。交配成功后第5天（种植窗期）取子宫组织，进行H&E染色、免疫组化和miRNA芯片检测。选取rno-miR-29c-3p, rno-miR-34c-5p, rno-miR-141-5p, rno-miR-24-1-5p, and rno-miR-490-5p做实时荧光定量PCR (qRT-PCR)验证。 结果：基因芯片发现内异症组和空白对照组内膜比较有475 miRNAs转录本表达异常，127个miRNAs表达上调，348个miRNAs表达下调。rno-miR-29c-3p, rno-miR-34c-5p, rno-miR-141-5p, rno-miR-24-1-5p, and rno-miR-490-5p的RT-PCR表达量在内异症组、脂肪组织对照组和空白对照组之间有明显表达差异。免疫组化结果示，ER 和PR在子宫内膜腺上皮和腺体当中的表达在三组之间有显著差异(P<0.05)。推测及5个miRNAs有可能参与生殖和着床。 结论：结果提示miRNAs、ER和 PR在内异症大鼠种植窗期有关键调控作用，miRNA有可能参与内异症内膜容受性的建立过程。.

[Abstract] [Full Text] [Related] [New Search]