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  • Title: Molecular cloning and characterization of the purE operon of Escherichia coli.
    Author: Kamholz J, Keyhani J, Gots JS.
    Journal: Gene; 1986; 44(1):55-62. PubMed ID: 3021590.
    Abstract:
    The purE operon of Escherichia coli has been cloned and localized to a 1.7-kb HpaI fragment. The operon has been further characterized by subcloning into the lac fusion vector, pMC1403, and by the construction of BAL 31-generated deletions. The purE regulation region has been identified by assay of beta-galactosidase produced by pur-lac fusion plasmids and by RNA polymerase binding to end-labelled restriction fragments. Two purE promoters have been identified; one strong that is regulated by purines, the other weaker which is not regulated. The latter may be internal to the purE1 structural gene.
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