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  • Title: A photoelectrochemical immunosensor based on gold nanoparticles/ZnAgInS quaternary quantum dots for the high-performance determination of hepatitis B virus surface antigen.
    Author: Hu Y, Huang Y, Wang Y, Li C, Wong W, Ye X, Sun D.
    Journal: Anal Chim Acta; 2018 Dec 04; 1035():136-145. PubMed ID: 30224131.
    Abstract:
    ZnAgInS quaternary quantum dots were prepared using glutathione as the capped reagent. Gold nanoparticles (GNPs) were integrated with ZnAgInS QDs to provide a GNPs/ZnAgInS QDs nanocomposite. The morphological image, component and crystal structure of GNPs/ZnAgInS QDs were characterized by transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD). A glassy carbon electrode surface was coated with GNPs/ZnAgInS QDs nanocomposites to construct an interface for immobilizing the antibody of hepatitis B virus surface antigen (anti-HBsAg). By employing GNPs/ZnAgInS QDs as a photoactive element, a photoelectrochemical immunosensor for hepatitis B virus surface antigen (HBsAg) was developed. The results indicate that gold nanoparticles can dramatically enhance the photocurrent response of ZnAgInS QDs and thus improving the sensing performances of the immunosensor. The experimental conditions including incubation time, incubation temperature, and ascorbic acid concentration were optimized. The relative photocurrent decline [Ri = ΔI/I0= (I0 - I)/I0] shows a linear relationship to the logarithm of HBsAg concentration [lg(c, ng mL-1)] in the range from 0.005 to 30 ng mL-1. A detection limit of 0.5 pg mL-1 was obtained. The immunosensor shows excellent sensitivity, selectivity, stability and reproducibility. The HBsAg concentrations in clinical serum samples were also accurately determined with this new photoelectrochemical immunosensor.
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