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  • Title: The metH gene from Salmonella typhimurium LT2: cloning and initial characterization.
    Author: Urbanowski ML, Stauffer GV.
    Journal: Gene; 1986; 44(2-3):211-7. PubMed ID: 3023184.
    Abstract:
    A 19-kb EcoRI DNA fragment carrying the metH gene of Salmonella typhimurium LT2 was cloned into plasmid vector pACYC184 and propagated in Escherichia coli K-12. The size of the metH gene product was observed to be approx. 120 kDa, as determined by SDS-polyacrylamide gel analysis of plasmid-specific polypeptides synthesized in a minicell system. The direction of transcription of the metH gene relative to the cloned fragment was determined, and the positions of translation initiation and termination were estimated.
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