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  • Title: Expression of the Epstein-Barr virus major membrane proteins in Chinese hamster ovary cells.
    Author: Motz M, Deby G, Jilg W, Wolf H.
    Journal: Gene; 1986; 44(2-3):353-9. PubMed ID: 3023198.
    Abstract:
    The gene of the major membrane antigen (gp250/350) of the Epstein-Barr virus (EBV) was isolated and inserted under the control of the SV40 early promoter into a eukaryotic expression vector, which allows selection for dhfr+ phenotype. Following transfection with this vector, Chinese hamster ovary cells express on their surfaces proteins immunologically similar to the major EBV membrane antigen. The transcript encoding gp250/350 is processed by partial splicing similarly but more efficiency than in B95-8 cells from which the DNA originates.
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