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  • Title: Exploration and application of a highly sensitive bis(salamo)-based fluorescent sensor for B4O72- in water-containing systems and living cells.
    Author: Pu LM, Li XY, Hao J, Sun YX, Zhang Y, Long HT, Dong WK.
    Journal: Sci Rep; 2018 Sep 19; 8(1):14058. PubMed ID: 30232405.
    Abstract:
    A highly selective fluorescent sensor H4L based on a bis(salamo)-type compound with two N2O2 chelating moieties as ionophore was successfully developed. Sensor H4L was found to have excellent selectivity for B4O72- over many other anions (Br-, CI-, CN-, CO32-, HCO3-, H2PO4-, HSO4-, NO3-, OAc-, S2O3-, SCN-, SO42-, Hcy (homocysteine) and H2O2), and it exhibited an approximately 150-fold enhancement of the fluorescence response to B4O72- in Tris-HCl buffer (DMF/H2O = 9:1, v/v, pH = 7) solutions. Significantly, its fluorescence intensity was enhanced in a linear fashion with increasing concentrations of B4O72-. The detection limit of sensor H4L towards B4O72- was 8.61 × 10-7 M. The test strips could conveniently, efficiently and simply detect B4O72- ions in Tris-HCl buffer (DMF/H2O = 9:1, v/v, pH = 7) solutions. Furthermore, sensor H4L showed excellent membrane permeability in living cells, and it was successfully used to monitor intracellular B4O72- by confocal luminescence imaging.
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