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  • Title: Binding of single-chain prourokinase to the urokinase receptor of human U937 cells.
    Author: Cubellis MV, Nolli ML, Cassani G, Blasi F.
    Journal: J Biol Chem; 1986 Dec 05; 261(34):15819-22. PubMed ID: 3023326.
    Abstract:
    The single-chain form of human urokinase plasminogen activator (uPA) is the major form of the enzyme found in cells, tissues, and extracellular fluids. The protein, called pro-uPA, has high (Kd = 0.5 nM) affinity for the specific uPA receptor of U937 human monocyte-like cells. Its conversion to two-chain uPA by plasmin does not appreciably change the binding parameters. In addition, conversion of pro-uPA to uPA occurs with receptor-bound pro-uPA and does not lead to dissociation from the membrane. These data show that secreted pro-uPA can find its way to the specific surface receptor without previous conversion to the two-chain form and that, once bound, can be activated by plasmin.
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