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  • Title: [Studies on the DNA damage in the transformed bronchial epithelial cells induced by hexavalent chromium].
    Author: Ren XH, Lu WX, Chen ZH, Liu W, Wang SQ, Luo NY, Liu JJ.
    Journal: Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi; 2018 Jul 20; 36(7):481-484. PubMed ID: 30248757.
    Abstract:
    Objective: To investigate DNA damage in the transformed human bronchial epithelial cells (16HBE) induced by hexavalent chromium (Cr(6+)) and further elucidate the potential carcinogenesis mechanism of Cr(6+). Methods: 16HBE were treated with different concentration of Cr(6+ ()0, 0.625, 1.25, 2.5 μmol/L) for 15 weeks. The malignant degrees of transformed cells were identified by the assays for anchorage-independent growth and tumorigenicity. According to the single cell gel electrophoresis (SCGE) assay, the DNA damage rate was calculated. The expression level of 53BP1 was determined by Western blot. Results: Chromium-treated cells could form colonies in soft agar and tumors in nude mice. Compared with the control group, colony formation efficiency of 1.25μmol/L and 2.5 μmol/L Cr(6+)-treated cells in soft agar showed significant increases (p<0.05) . The 2.5 μmol/L Cr(6+)-treated cells also formed tumors subcutaneously in nude mice. Cr(6+) could cause different degree of DNA damage to 16HBE cells in a dose-dependent manner. In addition, Western blot analyses showed that 53BP1 was aberrantly down-regulated at 2.5 μmol/L dose and has no significant changes at 0.625 μmol/L and 1.25 μmol/L dose under the treatment of Cr(6+). Conclusion: The declined expression of 53BP1 may mediate Cr(6+)-induced DNA damage and further involved in the cell malignant transformation. 目的： 通过分析六价铬Cr(6+)致16HBE细胞DNA损伤相关的恶性转化情况，为进一步研究Cr(6+)的毒性作用机制提供新思路。 方法： 以正常人支气管上皮细胞（16HBE）为研究对象，使用梯度浓度Cr(6+)（0、0.625、1.25、2.5 μmol/L）染毒处理，通过软琼脂克隆和裸鼠成瘤实验鉴定细胞恶性转化模型是否构建成功。同时，使用单细胞凝胶电泳实验检测细胞DNA损伤情况，并进一步利用免疫印迹（Western blot）法检测细胞DNA损伤修复关键蛋白53BP1的表达水平。 结果： 软琼脂克隆与裸鼠成瘤实验结果显示，Cr(6+)染毒16HBE细胞15周后，细胞恶性转化成功。1.25和2.5 μmol/L Cr(6+)处理组在软琼脂中的集落形成数明显高于对照组，且差异有统计学意义（P<0.05）。2.5 μmol/L Cr(6+)处理组细胞可在裸鼠体内成瘤。Cr(6+)染毒后会造成细胞不同程度的DNA损伤，且呈剂量效应关系。Western blot结果显示，与对照组比较，0.625 μmol/L和1.25 μmol/L浓度组53BP1的相对表达没有明显变化，2.5 μmol/L浓度组53BP1的相对表达明显低于对照组，且差异有统计学意义（P<0.01）。 结论： Cr(6+)可能通过抑制53BP1的DNA损伤修复水平导致细胞DNA损伤得不到及时修复进而诱导细胞恶性转化。.
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