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  • Title: Enhanced biocatalytic activity of immobilized steapsin lipase in supercritical carbon dioxide for production of biodiesel using waste cooking oil.
    Author: Badgujar VC, Badgujar KC, Yeole PM, Bhanage BM.
    Journal: Bioprocess Biosyst Eng; 2019 Jan; 42(1):47-61. PubMed ID: 30251191.
    Abstract:
    The present work reports covalent immobilization of steapsin lipase (SL) on Immobead-350 support matrix (IMB) to make a robust biocatalytic system to work under supercritical carbon dioxide condition (Sc-CO2). The developed biocatalyst (IMB:SL) was characterized in details and utilized to convert waste cooking sunflower oil (WCSO) into value-added energy chemical (biodiesel) in Sc-CO2. All reaction process parameters were optimized in detail which offered 86.33% yield of biodiesel from WCSO. The developed Sc-CO2 protocol is compared with the solvent-free conventional synthesis, which indicates almost twofold higher yield of biodiesel in Sc-CO2 media as compared to solvent-free condition. To extend the scope, we have tested fresh and waste cooking oils (WCO) from various sources, offering 81-94% yield of biodiesel. The biocatalyst activity was investigated in various parameters of supercritical condition to know the biocatalyst stability in Sc-CO2. Besides this, IMB:SL biocatalyst was effectively reused up to five recycle.
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