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  • Title: Comparative in depth RNA sequencing of P. tricornutum's morphotypes reveals specific features of the oval morphotype.
    Author: Ovide C, Kiefer-Meyer MC, Bérard C, Vergne N, Lecroq T, Plasson C, Burel C, Bernard S, Driouich A, Lerouge P, Tournier I, Dauchel H, Bardor M.
    Journal: Sci Rep; 2018 Sep 25; 8(1):14340. PubMed ID: 30254372.
    Abstract:
    Phaeodactylum tricornutum is the most studied diatom encountered principally in coastal unstable environments. It has been hypothesized that the great adaptability of P. tricornutum is probably due to its pleomorphism. Indeed, P. tricornutum is an atypical diatom since it can display three morphotypes: fusiform, triradiate and oval. Currently, little information is available regarding the physiological significance of this morphogenesis. In this study, we adapted P. tricornutum Pt3 strain to obtain algal culture particularly enriched in one dominant morphotype: fusiform, triradiate or oval. These cultures were used to run high-throughput RNA-Sequencing. The whole mRNA transcriptome of each morphotype was determined. Pairwise comparisons highlighted biological processes and molecular functions which are up- and down-regulated. Finally, intersection analysis allowed us to identify the specific features from the oval morphotype which is of particular interest as it is often described to be more resistant to stresses. This study represent the first transcriptome wide characterization of the three morphotypes from P. tricornutum performed on cultures specifically enriched issued from the same Pt3 strain. This work represents an important step for the understanding of the morphogenesis in P. tricornutum and highlights the particular features of the oval morphotype.
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