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  • Title: Griffipavixanthone induces apoptosis of human breast cancer MCF-7 cells in vitro.
    Author: Ma Y, Wang Y, Song B.
    Journal: Breast Cancer; 2019 Mar; 26(2):190-197. PubMed ID: 30259331.
    Abstract:
    BACKGROUND: Griffipavixanthone (GPX) is a compound extracted from Garcinia oblongifolia Champ. But, no research has yet been done about the effect of GPX on breast cancer. METHODS: We evaluated the proliferation of human breast cancer cells by CCK-8 assay and apoptosis by Annexin V (AV)-FITC and PI double staining. We used transwell assay to indicate the invasion and migration of MCF-7. To explore the molecular mechanism of GPX, we detected the mRNA and protein expression using qRT-PCR and Western blot. RESULTS: In this study, we evaluated if GPX could inhibit the proliferation of human breast cancer cell MCF-7 and T-47D with IC50 value of 9.64 ± 0.12 µM and 10.2 1 ± 0.38 µM at 48 h. And the IC50 value of MCF-10A is 32.11 ± 0.21 µM, which showed GPX had a tiny side effect for normal breast cells. Annexin V (AV)-FITC and PI double staining demonstrated firmly the apoptosis of MCF-7 resulting from GPX. Transwell assay indicated that GPX inhibited the invasion and migration of MCF-7. In addition, we found GPX cleaved caspase-8/9 and PARP, which play important roles in apoptotic pathway. Furthermore, through the Western blot assay, GPX increased the level of pro-apoptosis protein Bax and cytochrome C. On the contrary, GPX decreased the level of anti-apoptosis protein Bcl-2. Moreover, GPX increased the mRNA and protein expression level of p53 and its target genes, which indicated that GPX induced MCF-7 cell apoptosis by up-regulating p53 and Bax expression while suppressing Bcl-2 expression. CONCLUSION: All the results showed that GPX induces MCF-7 cell apoptosis and could be considered as a potential drug for breast cancer.
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