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  • Title: [Stimulation of arbovirus reproduction in cell cultures by hormones].
    Author: Kokorev VS, Kolotvinova EG.
    Journal: Vopr Virusol; 1986; 31(5):623-9. PubMed ID: 3026097.
    Abstract:
    The possibility of stimulation of reproduction and antigen production of arboviruses (tick-borne encephalitis, Japanese encephalitis, western equine encephalomyelitis) in cell cultures (SPEV, chick embryo fibroblasts) by using various hormones (insulin, ACTH, hydrocortisone) and optimal temperatures was demonstrated. The stimulating effect depended upon the type of cell culture, virus, method of cell treatment, and manifested by a significant increase of the hemagglutinating and infectious activity of the replicating viruses. Differences in the structure of populations, biophysical and antigenic properties of the viruses associated with the conditions of their reproduction in vitro were observed.
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