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  • Title: Ultrasound-assisted extraction of bioactive compounds from Malva sylvestris leaves and its comparison with agitated bed extraction technique.
    Author: Bimakr M, Ganjloo A, Zarringhalami S, Ansarian E.
    Journal: Food Sci Biotechnol; 2017; 26(6):1481-1490. PubMed ID: 30263685.
    Abstract:
    The effects of ultrasound-assisted extraction (UAE) variables-namely extraction temperature (40-60 °C), ultrasonic power (50-150 W), and sonication time (40-60 min)-on the extractive value (EV) of bioactive phenolics from Malva sylvestris leaves were investigated and optimized using Response surface methodology. The effects of extraction solvents (ethanol, ethyl acetate, and n-hexane) on EV, free radical scavenging activity (FRSA), total phenolic content (TPC), and major bioactive phenolics were studied using agitated bed extraction (ABE), and the results were compared with the UAE findings. Under the optimal UAE conditions (48 °C, 110.00 W, and 48.77 min) the experimental EV was 279.89 ± 0.21 mg/g with 71.12 ± 0.15% DPPHsc, 73.35 ± 0.11% ABTSsc, and a TPC of 152.25 ± 0.14 mg GAE/g. Ethanolic ABE results in higher EV (320.16 ± 0.25 mg g-1) compared to UAE, while the FRSA and TPC values were reduced. HPLC analysis revealed that the concentration of bioactive phenolics increased significantly (p < 0.05) under the optimal UAE conditions.
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