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  • Title: Detection of delta 9-THC in saliva by capillary GC/ECD after marihuana smoking.
    Author: Maseda C, Hama K, Fukui Y, Matsubara K, Takahashi S, Akane A.
    Journal: Forensic Sci Int; 1986 Dec; 32(4):259-66. PubMed ID: 3026938.
    Abstract:
    A method is described for the determination of delta 9-tetrahydrocannabinol (delta 9-THC) in the saliva by the use of a combination of moving-precolumn injector and glass capillary gas chromatograph with electron capture detector (GC/ECD). There were no interfering peaks due to impurities around the peak of pentafluoropropyl derivative of delta 9-THC (delta 9-THC-PFP). This GC/ECD method was linear over the range of 5-200 ng/ml of delta 9-THC-PFP. The lower detection limit was approximately 1 ng/ml. delta 9-THC content in the saliva after experimental marihuana smoking was measured by this method. It was demonstrated that for at least 4 h after smoking the level of delta 9-THC was sufficient for detection.
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