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  • Title: Demonstration of herpes simplex 1 virus antigen mouse brain after fixation.
    Author: Takimoto M, Sata T, Kurata T, Hondo R, Aoyama Y, Tashiro K.
    Journal: Jpn J Exp Med; 1986 Oct; 56(5):201-6. PubMed ID: 3027431.
    Abstract:
    The effects of several fixatives and fixation periods on recovery of viral antigen by indirect immunofluorescence (IF) and indirect immunoperoxidase (IP) were examined in mouse brains infected with type-1 herpes simplex virus. In 10% formalin, the sensitivity of antigen detection by IF decreased after one month fixation and viral antigen was barely detectable after fixation for three months. But in 10% buffered formalin antigenicity was not lost after six months. Similar effects were obtained by IP; however, specimens fixed in 10% formalin for three and six months showed positive reactions. Viral antigen was detectable without enzyme digestion in paraffin materials fixed in Bouin's, Carnoy's and Dubosq-Brazil solutions, but when treated with proteolytic enzyme for three hours, antigen could not be detected by either IF or IP. Ten per cent buffered formalin, Bouin's or Dubosq-Brazil solutions are recommended as fixatives for retrospective immunohistochemical and histopathological studies of viral infection in autopsy and biopsy materials.
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