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  • Title: Studies towards the development of chemically synthesized non-radioactive biotinylated nucleic acid hybridization probes.
    Author: Al-Hakim AH, Hull R.
    Journal: Nucleic Acids Res; 1986 Dec 22; 14(24):9965-76. PubMed ID: 3027670.
    Abstract:
    Non-radioactive nucleic acid hybridization probes have been constructed in which the reporter group is long chain biotin chemically linked to a basic macromolecule (histone H1, cytochrome C or polyethyleneimine). The modified basic macromolecule which carries many biotin residues can, in turn, be covalently linked to nucleic acids (DNA) via the bifunctional cross-linking reagents, glutaraldehyde, 1,2,7,8-diepoxyoctane, bis (succinimidyl) suberate or bis (sulfonosuccinimidyl) suberate. This provides a very sensitive probe by which as little as between 10-50fg of target DNA can be visualized using dot-blot hybridization procedures in conjunction with avidin or streptavidin enzyme conjugates.
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