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  • Title: MiR-940 inhibits the progression of NSCLC by targeting FAM83F.
    Author: Gu GM, Zhan YY, Abuduwaili K, Wang XL, Li XQ, Zhu HG, Liu CL.
    Journal: Eur Rev Med Pharmacol Sci; 2018 Sep; 22(18):5964-5971. PubMed ID: 30280778.
    Abstract:
    OBJECTIVE: We investigate whether miR-940 could target family sequence similarity 83 member F (FAM83F) and further inhibit the progression of non-small cell lung cancer (NSCLC). PATIENTS AND METHODS: The expression levels of miR-940 and FAM83F in tumor tissues and paracancerous tissues of 72 NSCLC patients were detected through quantitative real time-polymerase chain reaction (qRT-PCR). The relationship between their expression levels, tumor size, and prognosis of NSCLC was analyzed. Transfection plasmids were constructed to knockdown or overexpress miR-940 in H1299 cells (inhibitor group) and SK-MES-1 cells (mimic group). The viability of H1299 cells and SK-MES-1 cells was evaluated using cell counting kit-8 (CCK-8) assay after transfection. The combination of miR-940 and Ago2 was confirmed by RNA immunoprecipitation (RIP) experiment. The binding condition of miR-940 in FAM83F-WT and FAM83F-MUT groups was verified by luciferase reporter gene assay. RESULTS: MiR-940 expression was noticeably decreased, while FAM83F expression was distinctly upregulated in NSCLC tissues than that of paracancerous tissues. The overall survival rate of NSCLC patients with highly-expressed miR-940 was significantly higher than those with lowly-expressed miR-940. Besides, miR-940 level was negatively correlated with tumor stage and size of NSCLC patients. Knockdown of miR-940 evidently enhanced the activity of H1299 cells, while overexpression of miR-940 decreased the viability of SK-MES-1 cells. In addition, miR-940 was confirmed to combine with FAM83F. Luciferase activity of cells co-transfected with FAM83F-WT and miR-940 mimic was significantly decreased. CONCLUSIONS: MiR-940 inhibited the proliferation of cancer cells by targeting FAM83F and further restrained the progression of NSCLC.
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