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  • Title: Spin-trapping of sulfite radical anion, SO3-., by a water-soluble, nitroso-aromatic spin-trap.
    Author: Ozawa T, Hanaki A.
    Journal: Biochem Biophys Res Commun; 1987 Jan 30; 142(2):410-6. PubMed ID: 3028413.
    Abstract:
    Sulfite radical anion, SO3-., which is generated either by non-enzymatic reaction of hydrogen peroxide (H2O2-) with sulfite (SO3(2-)) or by the oxidation of bisulfite (HSO3) with Ce4+ ion, can be trapped with a water-soluble, nitroso-aromatic spin-trap, sodium 3,5-dibromo-4-nitrosobenzenesulfonate (DBNBS, 1), yielding an ESR spectrum with coupling constants [aN (1) = 12.9 G, aH (2) = 0.8 G] and a g-value of 2.0063. The SO3- radical adduct (spin adduct) was observed even in the presence of the very low concentration of H2O2 (1.21 X 10(-2) mumol).
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