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  • Title: Simultaneous light and electron microscopic observation of immunolabeled liver 27 KD gap junction protein on ultra-thin cryosections.
    Author: Dermietzel R, Yancey B, Janssen-Timmen U, Traub O, Willecke K, Revel JP.
    Journal: J Histochem Cytochem; 1987 Mar; 35(3):387-92. PubMed ID: 3029214.
    Abstract:
    We report on immunolabeling of gap junction protein in rat liver. Simultaneous light and electron microscopic immunolabeling of ultra-thin frozen sections was performed to confirm that the antigenic targets of polyclonal antibodies and a monoclonal 27 KD antibody (12/1 C5) are the gap junctions. Our results clearly demonstrate that the immunoreactive sites determined by indirect immunofluorescence correspond to immunogold-labeled gap junctions identified in the same section according to electron microscopic criteria. Our results also support the concept that the 27 KD protein is a major constituent of gap junctions.
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