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  • Title: Phosphorylation of brain muscarinic receptor: evidence of receptor regulation.
    Author: Ho AK, Ling QL, Duffield R, Lam PH, Wang JH.
    Journal: Biochem Biophys Res Commun; 1987 Feb 13; 142(3):911-8. PubMed ID: 3030306.
    Abstract:
    Muscarinic receptor, from porcine synaptic membrane, was purified by affinity chromatography. Molecular weight analysis by SDS-gel electrophoresis revealed one major peptide with an apparent Mr of 68 +/- 2 Kda. The purified receptor was phosphorylated by the catalytic subunit of cAMP-dependent protein kinase resulting in a concomitant loss in specific binding, and this loss was reversed by calcineurin.
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