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  • Title: A quantitative relationship between Cl- enhanced [3H]flunitrazepam and [35S]t-butylbicyclophosphorothionate binding to the benzodiazepine/GABA receptor chloride ionophore complex.
    Author: Havoundjian H, Skolnick P.
    Journal: Brain Res; 1986 Dec; 387(3):281-7. PubMed ID: 3030489.
    Abstract:
    A quantitative relationship between the efficacy (i.e. maximum enhancement) of Cl- to increase [3H]flunitrazepam binding and the density of [35S]t-butylbicyclophosphorothionate binding sites was observed in well-washed membrane fragments of rat cerebral cortex previously exposed to phospholipase A2. This relationship (described by the equation y = ABx) was maintained when [3H]flunitrazepam was assayed at Cl- concentrations between 100 and 600 mM, and was not qualitatively altered by the presence of 100 microM pentobarbital. However, under experimental conditions that reduced the ratio of [35S]TBPS binding sites/benzodiazepine receptors, the effects of pentobarbital suggest that the conductance state of benzodiazepine receptor-coupled chloride channels may be the primary determinant of Cl- enhanced [3H]flunitrazepam binding.
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