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  • Title: [Electrophoretic and immunologic characteristics of the Fc+ and Fc- fractions of the splenic cells of CBA mice].
    Author: Malaĭtsev VV, Bogdanova IM.
    Journal: Biull Eksp Biol Med; 1977 Oct; 84(10):451-4. PubMed ID: 303124.
    Abstract:
    Conditions have been established for the effective separation of Fc+ and Fc- cells, using sheep red blood cells monolayer, sensitized with the anti-SRBC antibodies. The method was modified by replacement of poly-L-lysine by protamine sulfate. The electrophoretic distribution profiles of Fc+ and Fc- mouse splenocytes were determined. Fc+ cells had mostly a slow electrophoretic mobility. The Fc- cells were more mobile. The Fc+ fraction contained mainly B-lymphocytes, and the Fc- fraction was enriched with T cells. There was an extensive overlap between the electrophoretic profiles of the Fc+ and Fc- splenocytes. After the splenocyte passage through the glass bead column the overlap became much less.
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