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  • Title: Two dimensional gel analysis of HLA-DR2 associated HLA-D clusters.
    Author: Nakatsuji T, Moriya K, Tsuji K.
    Journal: Tokai J Exp Clin Med; 1986 May; 11(2):125-31. PubMed ID: 3031855.
    Abstract:
    Five cell lines with different cellular HLA-D specificities associated with HLA-DR2, i.e. Dw2, Dw12, MN2, DB9 and D2J, displayed structural variation in the products of the DR and DQ loci. Analyses of immunoprecipitated DR molecules from HLA-D homozygous B-lymphoblastoid cell lines by two dimensional polyacrylamide gel electrophoresis showed variable patterns with one set of DR beta chains. Another set of DR beta chain products, however, was homogeneous in all cell lines studied. In addition, analyses of DQ molecules showed extremely variant DQ beta chains, and slightly variant DQ alpha chains.
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