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  • Title: Fluorescein mercuric acetate specifically displaces zinc from cytochrome oxidase.
    Author: Moubarak A, Pan LP, Millett F.
    Journal: Biochem Biophys Res Commun; 1987 Mar 30; 143(3):1030-6. PubMed ID: 3032179.
    Abstract:
    Treatment of beef heart cytochrome oxidase with fluorescein mercuric acetate (FMA) was found to specifically displace zinc from the enzyme and inhibit the steady-state activity in a parallel fashion. The native cytochrome oxidase preparation contained 2.3 Cu: 2.0 Fe: 1.1 Zn: 0.9 Mg. Addition of 2 equivalents of FMA inhibited the activity by 50% and displaced 60% of the zinc from the enzyme, but did not affect the copper, iron or magnesium content. The pre-steady-state reduction of cytochrome oxidase by ferrocytochrome c was not affected by the FMA treatment, in contrast to the inhibition of steady state activity. These results suggest a possible structural or functional role for zinc in cytochrome oxidase.
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