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  • Title: The effect of polymyxin B nonapeptide (PMBN) on transformation.
    Author: Viljanen P.
    Journal: Biochem Biophys Res Commun; 1987 Mar 30; 143(3):923-7. PubMed ID: 3032189.
    Abstract:
    The effect of the outer membrane permeabilizing polycation, polymyxin B nonapeptide (PMBN) on the transformation of E. coli HB101 with pBR322 plasmid DNA was investigated. Pretreatment of cells with PMBN (followed by suspending the cells in PMBN-free medium) did not stimulate the development of competence induced by the calcium heat pulse. In the absence of calcium-ions, a high PMBN concentration (1 mM) was able to induce a low transformation frequency provided that PMBN was not removed before the addition of DNA.
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