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  • Title: [Growth of neurites and formation of connections in cultures of pteropodial mollusc neurons].
    Author: Arshavskiĭ IuI, Gel'fand IM, Deliagina TG, Orlovskiĭ GN, Pavlova GA.
    Journal: Neirofiziologiia; 1987; 19(1):81-6. PubMed ID: 3033527.
    Abstract:
    Dissociated neurons from the brain of pteropodial mollusc were cultivated in a 25% Leibovitz medium containing 2% of calf serum. Neurite outgrowth was observed in 1-30% of the neurons. It was maximum during the first 3 days. Neurite length reached 300 microns. Membrane potential of neurons was 40-60 mV; they generated single spikes or bursts of impulses. Intercellular connections were tested on the 3-4th days in 70 pairs of neurons with neurites overlapped. Electrical connections between the cells were observed in 20% of the pairs tested, and in 6% of pairs stimulation of one of the neurons evoked an inhibitory postsynaptic potential in the other.
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